LOCUS       40924_31640       10455 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB300 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 10455)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10455)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(301..357)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      362..614
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(648..664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    672..688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(696..726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(741..762)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(847..1022)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1030..3066)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49874.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(1030..3066)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(3106..3782)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(3977..4001)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4522..5310
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5559..6147
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6333..6473)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6817..7011)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7080..8150)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8582..9208)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaatttccc
      361 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
      421 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
      481 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
      541 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
      601 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatggtc atagctgttt
      661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc
      781 tatttctgaa ttcaacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
      841 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
      901 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
      961 aaaccctata ggaaccctaa attcccttat ctgggaacta ctcacacatt attatggaga
     1021 aactcgaagt tagtatatag tccttccatc accctctgtg ataatctcct taaaacttgc
     1081 tcccccagga atcataggca gaacgtgctc ctggtgggga actatcacat ccagtaaata
     1141 aggaccggga gaatctagca tagtttgaat tgctgctctt agttcatcct tcttcaccac
     1201 acgcgcattt ggcaccttga aaccgtctgc aactgtacag aagtcggggt agatctgctc
     1261 ctcgtgatct ggatcaccta agtaagtatg tgctctgttg gccttgtaga atctatcctc
     1321 caattgtacg accattccta gatgttggtt gtttaagatc atgatcttca cgggtaactt
     1381 ctccacaaaa attgtagcca actcctgtac gttcatcaca aagctaccat cgccatcgat
     1441 gtcgatcaca ggaatatctg gttgagttgc ggcagccccc aaagcagaag gaagtccgaa
     1501 tcccatggct ccaagacctc cagatgtcaa ccaacgccgc ggttcgttgt acttgaacca
     1561 ctgggctgcc cacatttgat gttggccaac accagtagat atgatggcat ttccaccagt
     1621 gagttcgtac agcgtctgta tggcagattg aggagcaatg gcatcctcga atacgggaaa
     1681 cttcattggc catttctgct tttgttcttc tagttctgcc acccagttgg agaagtcgaa
     1741 agtcctcttt tgactcttca agagggcatt gatttcactt agcgcaggtt tcacatcagc
     1801 acacatggac acatggggct gtttgttctt tccaatttca gcagggtcaa tatcgatatg
     1861 aacgatgctg gacctgctag caaaagtctc cagtttacca gtcactcgat cgtcgaaacg
     1921 cactccaaat gccaacaaca gatctgcttg gtccacggcg tagttggcgt aaacagttcc
     1981 atgcattcca agcattttca aacttaatgg atcagactcg ggatacgctc ccaaacccat
     2041 taaagtcgat gtgataggaa ttccggtaag ctcgacaaat tccctcaact cttcgctggc
     2101 gtgattgcaa cctcctccag aatacactac aggcttctta gaactagaaa tgagtcttat
     2161 aatctgctcg attaacgttg gttccggtgg ccgaggcagt cgttgtaagt agcccttcag
     2221 ttgcattggc tgagaccaat ctggaatagc catttgctgt tgcacgtcct tgggaatatc
     2281 taccaagact gggcccggcc tacctgaagc tgccaaaaag aaagcctccc tgattacgcg
     2341 agggatgtca tcaatgttca taacaaggta attgtgcttg gtgatcgatc tcgtaacctc
     2401 cacaattgga gtttcttgaa aagcatcggt accaatcatc ctactggata cttgacctgt
     2461 aatggccact agtggcacac tgtccaagag ggcatcagcg agcccggtaa caagattggt
     2521 cgctccagga ccggatgtgg ctatacaaac ccctactcgt ccagacgatt tagcataacc
     2581 ttcagcagcg aaaatctccc cctgctcgtg gcgacagaga atgttgcgga tgctgctgga
     2641 acgtgtaaga gcctggtgaa tctccataga cgctccgccg ggataagcga acaccgttct
     2701 aatcccttct cgctcaagag cctccaccaa aatgtcggct cctttgcggg gctcgtcatc
     2761 cttgtagcga gactgaaagt cggacggggt tggtgcaacg agtgacttcc cgctgctaag
     2821 ccgtggtcca ctcacatcga ctgcagactg gatcttctcg ctaacagctt gggctttcac
     2881 ttccagtgaa cgctcagtct tccgagaggt gttgtatgca tgtcccctca gagcggctcc
     2941 cgagaagaaa ccactcctca cctttagctc tttagctccc ttgcaacaag accttgccga
     3001 tggctgcacc ttctccaggc tcgctatgct caaaggcaca gctgcgcatc cactaattga
     3061 cgccatgctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     3121 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     3181 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     3241 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     3301 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa ttatggcatt tgtaggtgcc
     3361 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     3421 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     3481 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
     3541 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     3601 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     3661 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     3721 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     3781 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     3841 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgaatt cagaaataaa
     3901 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
     3961 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
     4021 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
     4081 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
     4141 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
     4201 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg
     4261 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     4321 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     4381 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     4441 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     4501 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta
     4561 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     4621 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     4681 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     4741 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     4801 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     4861 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     4921 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     4981 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     5041 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     5101 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     5161 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     5221 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     5281 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg
     5341 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca
     5401 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta
     5461 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5521 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5581 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5641 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5701 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5761 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5821 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5881 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5941 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6001 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6061 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6121 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6181 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6241 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6301 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     6361 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     6421 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     6481 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6541 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6601 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct
     6661 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc
     6721 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct
     6781 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta
     6841 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata
     6901 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg
     6961 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga
     7021 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag
     7081 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca
     7141 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg
     7201 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc
     7261 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa
     7321 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc
     7381 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc
     7441 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc
     7501 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt
     7561 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag
     7621 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct
     7681 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa
     7741 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc
     7801 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg
     7861 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct
     7921 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc
     7981 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct
     8041 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca
     8101 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct
     8161 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg
     8221 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt
     8281 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc
     8341 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg
     8401 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc
     8461 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc
     8521 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc
     8581 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct
     8641 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct
     8701 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac
     8761 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat
     8821 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt
     8881 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca
     8941 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca
     9001 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca
     9061 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc
     9121 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt
     9181 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca
     9241 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
     9301 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
     9361 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
     9421 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
     9481 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
     9541 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
     9601 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     9661 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     9721 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     9781 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     9841 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     9901 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     9961 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
    10021 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
    10081 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
    10141 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
    10201 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
    10261 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
    10321 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
    10381 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
    10441 cgaacggata aacct
//
